Overview

Genohub provides researchers with easy access to sequencing and library prep services from a variety of service providers. By leveraging our database of instrument specs such as read length and numbers of reads per run, Genohub creates optimized sequencing packages based upon your project requirements. Genohub also facilitates the entire order process from shopping and project submission to order tracking, communication, and receiving results. The typical process is as follows:

Find a service → See package details → Enter project details and payment info → Submit project → Wait for confirmation → Ship samples/libraries → Receive results

NGS Matching Engine

If you know your required sequencing coverage or number of reads per sample, use our NGS Matching Engine to enter the specifications listed below and identify services that meet those requirements. You’ll be able to comparison shop, download quotes if you need to, and immediately submit your project.

Number of Samples

Enter the number of nucleic acid samples or libraries you’d like to sequence.

Application

Choose the application type for the library preparation you’d like performed by the service provider, or the type of your libraries if you're constructing them yourself.

Service

Choose whether you’d like to order a Sequencing and Library Prep service (you have nucleic acid samples to send to the provider), a Sequencing only service (you have pre-constructed libraries to send to the provider) or a Library Prep only service (you have nucleic acid samples to send to the provider, but don’t require sequencing).

Reads or Coverage per Sample

You can identify next-gen sequencing services that best match your project by entering a required minimum reads or coverage per sample. If you order by coverage (e.g. for 50x coverage enter 50) also enter the haploid genome size of the sample you are trying to sequence (e.g. 3 billion for the human genome). By leveraging our database of instrument specs such as read length and numbers of reads per run, Genohub is able to present you with sequencing packages that meet your minimum read number or coverage requirements.

Using the “Shop by Project” page, Genohub assumes that each sample or library you submit will receive an equal allocation of reads or coverage. For example, if your project contains 10 samples and you enter a requirement of 100 million reads, each of your samples will receive at least 10 million reads.

Indexing Samples for Multiplexed Sequencing

If you are submitting multiple samples or libraries and require that they be multiplexed, you must tell the provider using the sample and library submission sheet how you would like your libraries pooled. If you require an uneven sample allocation of reads or coverage, you should also describe this to the provider. Note that packages presented on Genohub assume an even allocation of reads and coverage per sample.

Sequencing Packages

Based on your entries above, Genohub generates packages using our database of instrument specs and displays the number of sequencing units needed to meet your read number and coverage requirements. Each sequencing package contains the following information:

• Name of service provider’s institution
• Country
• Max Turnaround time
• Instrument
• Coverage per sample (displayed only if searching by coverage)
• Output per sample
• Reads per sample
• Read length and whether its single read (SR) or paired end (PE)
• Allocation (number of sequencing units, e.g. flow cell lane, chip)
• Whether bioinformatic analysis is available
• Library application (if library prep is ordered)
• Library kit name (if library prep is ordered)

Allocation

Allocation refers to the number of sequencing units (flow cell lane, chip, SMRT cell) required to meet your entire project’s minimum number of reads or coverage. Genohub displays sequencing packages using a calculation that assumes you want an equal distribution of reads or coverage for each of your samples or libraries. If this is not the case, you will need to describe your uneven allocation requirement to the service provider using the standard sample and library prep submission spreadsheet

Sequencing Output

The number of reads per unit that Genohub uses to estimate coverage and output are based on the instrument manufacturer’s specs. A note of caution, these are estimates, not guarantees.

If the number of reads or coverage you require per sample is less than the output of a single sequencing unit, some packages may have output quantities that significantly exceed your requirements. A sequencing unit is the smallest allocation of a sequencer that can be priced. Below are the platform-specific definitions of sequencing unit:

• Illumina and SOLiD: 1 unit = 1 lane (single run)
• IonTorrent: 1 unit = 1 chip (single run)
• Roche 454: 1 unit = a single run

More reads means that you’ll have higher coverage. Guidance on coverage can be found in outSequencing Guide.

Filters

After you submit your requirements on the project page and start reviewing packages, you will have the option to narrow down your package choices using filters on the left hand column. These filters include:

• Platform
• Read type (single read or paired end)
• Read length
• CLIA certification
• Whether bioinformatic data analysis is available
• Packages immediately available for purchase

Read type

Several instruments allow you to read a fragment or sequence in both directions. You can specify this by choosing “single read” or “paired end” on the read type filter. More details on when you would choose one over the other can be found in the sequencing guide page .

Read Length

Genohub allows you to filter sequencing packages according to your desired sequencing length (this correlates to your sample’s nucleic acid fragment length). Just enter the minimum and maximum read length you are looking for and the appropriate packages will be displayed. Note that for paired-end (PE) read types, the read length must be multiplied by 2. For example if you're interested in paired-end reads of between 100 and 200 bases you must enter 200 and 400 for the min and max read length respectively. Guidance on read length can be found in our the sequencing guide page.

Additional Options

These are additional filters meant to narrow down packages that are more specific to your needs.

• Available for purchase
• CLIA certified
• Bioinformatic support available

Selecting the “Available for Purchase” filter will only display services that you can immediately order. If you do not select this filter you may find “Inquire” packages that require you to contact the provider for more information.

Choosing the CLIA certified packages will only display services from facilities that have CLIA certification.

Selecting “Bioinformatic support available” will only display packages from providers that offer bioinformatic data analysis. Look at the details of each package to determine if analysis support is included as part of your sequencing package or if it is available for an additonal charge.

Genohub Project Policies

All projects you start on Genohub are subject to Policies for Genohub-Managed Projects.