NGS Library Preparation Applications and Kits

⬅️ NGS Handbook

Overview

NGS library construction involves preparing nucleic acids targets using a coordinated series of enzymatic reactions to produce a random collection of DNA fragments, of specific size, for high throughput sequencing. Advances and the development of new library preparation technologies have expanded the application of next-generation sequencing to fields such as transcriptomics and epigenetics. Techniques such as chromatin immunoprecipitation, microRNA expression analysis and PCR based mutation detection, have now been enhanced with high throughput sequencing based protocols: ChIP-Seq, Small RNA-Seq and Amplicon-Seq.

Improvements in sequencing technologies have also required changes and improvements to library preparation. Next generation sequencing library preparation kits, developed by companies such as Agilent, Bioo Scientific, Kapa Biosystems, New England Biolabs, Illumina, Life Technologies, Pacific Biosciences and Roche not only add consistency and reproducibility to standard molecular biology reactions, but also enable access to new preparation strategies and ensure compatibility with the latest NGS instrument technology.

Whether you construct your own next generation sequencing library or plan on outsourcing it to a service provider, it is important to understand the fundamental processing steps of each library preparation kit or protocol prior to sequencing. This guide is an overview of library preparation applications and kits that are in common use for next-generation sequencing. We will be continually adding details on more library applications and kits to this page. There are a variety of NGS providers on Genohub who offer library preparation services using the kits below. We'd be happy to help you identify the right library construction and sequencing solution for your projects. Just fill out our Free Consultation Form and one of our staff scientists will get in touch with you.

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Exome Sequencing

Read more about exome sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available Illumina Exome library prep services, or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Agilent HaloPlex (for the Illumina platform)
Agilent SureSelect (for the Illumina platform)
Agilent SureSelect QXT (for the Illumina platform)
IDT xGen (for the Illumina platform)
Illumina Nextera Rapid Capture (for the Illumina platform)
Illumina TruSeq (for the Illumina platform)
MYcroarray MYbaits (for the Illumina platform)
Roche Nimblegen SeqCap (for the Illumina platform)


DNA-Seq (Whole Genome) Sequencing

Read more about DNA sequencing and the current kits and protocols for this next-generation sequencing application.

See the up-to-date list of available library prep services on Genohub for the following applications:

Illumina Whole Genome DNA Seq library prep
Illumina DNA Hydroxymethylation library prep
Ion DNA library prep services
PacBio DNA template prep services

Or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing, Ion PGM/Proton sequencing, PacBio sequencing.


Beckman SPRIworks Fragment Library (for the Illumina platform)
Beckman SPRIworks HT (for the Illumina platform)
Bioo Scientific NEXTflex DNA (for the Illumina platform)
Bioo Scientific NEXTflex PCR-Free (for the Illumina platform)
Bioo Scientific NEXTflex Rapid DNA (for the Illumina platform)
Illumina Nextera DNA (for the Illumina platform)
Illumina Nextera XT (for the Illumina platform)
Illumina TruSeq DNA (for the Illumina platform)
Illumina TruSeq DNA PCR-Free (for the Illumina platform)
IntegenX PrepX ILM DNA (for the Illumina platform)
Kapa DNA Library (for the Illumina platform)
Life Tech Ion Plus Fragment (for the Ion platform)
NEB NEBNext DNA (for the Illumina platform)
NEB NEBNext Ultra DNA (for the Illumina platform)
NEB Next Ultra II Directional DNA (for the Illumina platform)
NuGEN Encore Rapid Library (for the Illumina platform)
PacBio DNA Template Prep (for the PacBio platform)


Target Capture Gene Panels

Read more about Target Capture Gene Panels kits and applications.

See the up-to-date list of available library prep services on Genohub for Target Capture Gene Panels:

Illumina Target Capture Gene Panels


Agilent ClearSeq (for the Illumina platform)
Agilent SureSelect Capture (for the Illumina platform)
Archer FusionPlex (for the Illumina platform)
Bioo Scientific NEXTflex (for the Illumina platform)
IDT xGen (for the Illumina platform)
Illumina TruSight (for the Illumina platform)
Nimblegen SeqCap (for the Illumina platform)
Qiagen GeneRead (for the Illumina platform)


ChIP-Seq

Read more about ChIP sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available Illumina ChIP-Seq library prep services, or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Bioo Scientific NEXTflex ChIP (for the Illumina platform)
Diagenode iDEAL ChIP (for the Illumina platform)
Epigentek EpiNext ChIP (for the Illumina platform)
Illumina TruSeq ChIP (for the Illumina platform)


RNA Sequencing (mRNA or cDNA sequencing)

Read more about RNA-Seq and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available library prep services for the following applications:

Directional RNA (polyA-selected) Illumina library prep services
Directional RNA (rRNA-depleted) Illumina library prep services
RNA (polyA-selected) Illumina library prep services
RNA (rRNA-depleted) Illumina library prep services

If you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Bioo Scientific NEXTflex Rapid Directional qRNA (for the Illumina platform)
Bioo Scientific NEXTflex Rapid Directional RNA (for the Illumina platform)
Bioo Scientific NEXTflex Rapid Directional RNA (for the Illumina platform)
Bioo Scientific NEXTflex Rapid qRNA (for the Illumina platform)
Clontech SMARTer (for the Illumina platform)
Epicentre ScriptSeq (for the Illumina platform)
Epicentre ScriptSeq (for the Illumina platform)
Gnomegen RNA Profiling Kit (for the Illumina platform)
Illumina TruSeq Stranded (for the Illumina platform)
NEB NEBNext Ultra Directional RNA (for the Illumina platform)
NEB NEBNext Ultra Directional RNA (for the Illumina platform)
NEB Next Ultra II Directional RNA (for the Illumina platform)
NEB Next Ultra II Directional RNA (for the Illumina platform)


Small RNA Sequencing (microRNA-Seq)

Read more about small RNA sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available Illumina Small RNA (microRNA) library prep services, or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Bioo Scientific NEXTflex Small RNA Seq v2 (for the Illumina platform)
Epicentre ScriptMiner (for the Illumina platform)
Illumina TruSeq Small RNA (for the Illumina platform)
NEB NEBNext Small RNA (for the Illumina platform)
Seqmatic TailorMix miRNA Kit v2 (for the Illumina platform)


Metagenomics Sequencing

Read about coverage, read length and workflow recommendations for metagenomics sequencing applications

See Genohub's up-to-date list of available Illumina Metagenomics Sequencing Services



16S Amplicon Sequencing

Read more about 16S rRNA sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available Illumina 16S Amplicon-Seq library prep services, or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Bioo Scientific NEXTflex 16S V1-V3 Amplicon (for the Illumina platform)
Bioo Scientific NEXTflex 16S V4 Amplicon (for the Illumina platform)
Bioo Scientific NEXTflex 18S ITS Amplicon (for the Illumina platform)


Mate-Pair Sequencing

Read more about Mate-Pair sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available library prep services for the following applications:

Illumina mate pair (1-6kb) library prep services
Illumina mate pair (7-15kb) library prep services
Illumina mate pair (16-20kb) library prep services

If you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Illumina Nextera Mate Pair (for the Illumina platform)
Illumina Nextera Mate Pair (for the Illumina platform)


Methyl DNA Sequencing

Read more about Methly DNA sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available library prep services for the following applications:

Bisulfite-seq library prep services
RRBS library prep services
MeDIP library prep services
Targeted bisulfite library prep services

Bioo Scientific NEXTflex Bisulfite (for the Illumina platform)
Bioo Scientific NEXTflex Methyl-Seq (for the Illumina platform)
Roche Nimblegen SeqCap Epi Kit (for the Illumina platform)


Single Cell DNA-Seq

Read more about single cell DNA sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available Illumina Single Cell DNA library prep services, or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Qiagen REPLI-g Single Cell (for the Illumina platform)
Rubicon Genomics PicoPlex (for the Illumina platform)


Single Cell RNA-Seq

Read more about single cell RNA sequencing and the current kits and protocols for this next-generation sequencing application.

See Genohub's up-to-date list of available Illumina Single Cell RNA library prep services, or if you're doing your own library preparation see the list of facilities that offer Illumina sequencing.

Clontech SMARTer Ultra Low Input (for the Illumina platform)


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