⬅️ NGS Handbook
NGS library construction involves preparing nucleic acids targets using a coordinated series of
enzymatic reactions to produce a random collection of DNA fragments, of specific size, for high
throughput sequencing. Advances and the development of new library preparation technologies have
expanded the application of next-generation sequencing to fields such as transcriptomics and
epigenetics. Techniques such as chromatin immunoprecipitation, microRNA expression analysis and
PCR based mutation detection, have now been enhanced with high throughput sequencing based
protocols: ChIP-Seq, Small RNA-Seq and Amplicon-Seq.
Improvements in sequencing technologies have also required changes and improvements to library
preparation. Next generation sequencing library preparation kits, developed by companies such as
Agilent, Bioo Scientific, Kapa Biosystems, New England Biolabs, Illumina, Life Technologies,
Pacific Biosciences and Roche not only add consistency and reproducibility to standard molecular
biology reactions, but also enable access to new preparation strategies and ensure compatibility
with the latest NGS instrument technology.
Whether you construct your own next generation sequencing library or plan on outsourcing it to a
service provider, it is important to understand the fundamental processing steps of each library
preparation kit or protocol prior to sequencing. This guide is an overview of library
preparation applications and kits that are in common use for next-generation sequencing. We will
be continually adding details on more library applications and kits to this page. There are a
variety of NGS providers on Genohub who offer library preparation services using the kits below.
We'd be happy to help you identify the right library construction and sequencing solution for
your projects. Just submit a request on our main
page and one of our staff scientists will get in touch with you.
Read more about Target Capture Gene Panels kits and applications.
See the up-to-date list of available library prep services on Genohub for Target Capture Gene
Target Capture Gene Panels