Genome-wide optical maps can be constructed using restriction enzymes to cut at specific sites along
the DNA of organisms. These optical maps are unique DNA fingerprints that can be obtained from
single DNA molecules.
Using BioNano Optical Mapping and Data Solution services, you can obtain the most complete
genome assemblies and identify large-scale structural variations.
The BioNano Irys Instrument reveals genome structure by performing low-cost next-generation mapping
at single-molecule resolution. Irys provides the long-range genomic information required for
high-quality de novo assembly and high-resolution discovery of structural variations.
The BioNano Saphyr Instrument is a structural variation discovery platform. Saphyr detects
structural variations (insertions, deletions, translocations etc.) ranging from 1,000 bp to megabase
pairs in length using assembly and discovery algorithms.
You can use these next-generation mapping tools along with next-generation sequencing,
especially for building reference genomes. Using the Irys System, researchers have assembled
reference genomes for hummingbirds and the duck-billed platypus and have been able to increase the
reference genome diversity in humans.
Another important application of physical genome mapping is to detect structural variations. This is
due to the fact that unlike short-read sequencing technologies, optical genome mapping is not
limited by size and can look at much larger and more complex regions to identify variants. Thus,
whole-genome optical mapping can be a useful complementary tool to NGS for observing large complex
rearrangements and structural variations.
If you're interested in optical mapping services, please send us a quick email at projects@genohub.com
and tell us about your project. We can help you define the scope of the project and quickly get you
one or more quote(s) from our network of partnering service providers.