Complete Genomics DNBSEQ-T1+

DNBSEQ-T1+ is a sequencer released by Complete Genomics in September 2025. It is based on DNA Nanoball Sequencing.

Complete Genomics DNBSEQ-T1+
Photo of DNBSEQ-T1+: https://www.completegenomics.com/products/sequencing-platforms/dnbseq-t1-plus/ from Complete Genomics

DNBSEQ-T1+ is available in the following models:
  • DNBSEQ-T1+ FCL
  • DNBSEQ-T1+ FCM
  • DNBSEQ-T1+ FCS

Specifications

Manufacturer Complete Genomics
Technology DNA Nanoball Sequencing
Release date September 2025
Price [2]
Type Benchtop
Weight 295 Kg
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length Complete Genomics DNBSEQ-T1+ FCL: 1x100bp to 2x150bp
Complete Genomics DNBSEQ-T1+ FCM: 1x100bp to 2x150bp
Complete Genomics DNBSEQ-T1+ FCS: 1x100bp to 2x300bp
Run time Complete Genomics DNBSEQ-T1+ FCL: 10 to 23 hours
Complete Genomics DNBSEQ-T1+ FCM: 9 to 21.5 hours
Complete Genomics DNBSEQ-T1+ FCS: 8 to 36 hours
Reagent cost per Gb [1] Complete Genomics DNBSEQ-T1+ FCL: Unavailable
Complete Genomics DNBSEQ-T1+ FCM: Unavailable
Complete Genomics DNBSEQ-T1+ FCS: Unavailable
Data quality [3] Complete Genomics DNBSEQ-T1+ FCL: ≥ 90% bases above Q40 for PE150 or shorter
Complete Genomics DNBSEQ-T1+ FCM: ≥ 90% bases above Q40 for PE150 or shorter
Complete Genomics DNBSEQ-T1+ FCS: ≥ 90% bases above Q40 for PE150 or shorter
Maximum output per run (Gb)[4] Complete Genomics DNBSEQ-T1+ FCL: 600.00
Complete Genomics DNBSEQ-T1+ FCM: 300.00
Complete Genomics DNBSEQ-T1+ FCS: 300.00
Primary error type [5] Substitutions
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the Complete Genomics DNBSEQ-T1+

The DNBSEQ-T1+ is a mid- to high-throughput next-generation sequencing (NGS) instrument from MGI (a subsidiary of BGI Group). It provides researchers with a versatile and efficient platform for a wide range of genomic applications. Positioned as an advancement within the DNBSEQ series, it offers improved speed and integrated features compared to earlier mid-throughput models. It remains a current offering as of mid-2025, sitting below the ultra-high-throughput DNBSEQ-T7 and T20x2 systems.

Key Innovations and Features

The DNBSEQ-T1+ incorporates several key innovations to enhance throughput, accuracy, and user experience.

  • Dual Flow Cell Concurrent Operation: A major innovation is the ability to run two flow cells simultaneously, of which there are three types: FCS (small), FCM (medium), and FCL (large). This significantly boosts output, workflow efficiency, and versatility.
  • Integrated DNB Make & Load Module: This module streamlines the workflow by preparing and loading DNA Nanoballs (DNBs) directly on the sequencer. Traditionally, these steps are performed offline with separate equipment. Integrating these processes simplifies operation, reduces hands-on time, and minimizes potential errors and contamination.
  • Optional Built-in Bioinformatics: The DNBSEQ-T1+ARS version features a built-in bioinformatics module that automates primary data analysis, such as FASTQ file generation while also supporting secondary advanced analysis, accelerating the time from sample to analysis.
  • High-Quality Data: MGI reports high-quality metrics, with ≥90% of bases achieving Q40 for paired-end 150 bp (PE150) or shorter reads across all flow cell types. The DNBSEQ technology itself contributes to this by minimizing amplification errors, indel errors, and index hopping.
  • Individually Addressable Lanes: Each flow cell is equipped with independent lanes, allowing different sequencing read lengths or application types to be run concurrently within the same flow cell.

Features and Technologies

The DNBSEQ-T1+ utilizes DNA Nanoball (DNB) sequencing or DNBSEQ. This technology uses a different approach from other sequencing-by-synthesis platforms, particularly those that use bridge amplification.

  1. DNA Nanoball Generation: The process begins with library preparation where double-stranded DNA fragments are converted into single-stranded circular DNA (ssCirDNA). These circular templates then undergo Rolling Circle Amplification (RCA), creating a single DNB with hundreds of copies of the original DNA circle. This method is designed to reduce amplification errors and PCR-induced biases.
  2. Patterned Array Flow Cells: The DNBs are loaded onto a patterned array flow cell. These flow cells have a precisely etched pattern of binding sites, each designed to hold a single DNB. This ensures high data density and minimizes signal interference.
  3. Combinatorial Probe-Anchor Synthesis (cPAS): The sequencing uses a proprietary cPAS chemistry. In each cycle, a sequencing primer binds to the DNB, and fluorescently labeled probes are incorporated by a DNA polymerase. After detection, the fluorescent signal and 3' terminator are cleaved, allowing the next base to be added. The DNBSEQ-T1+ also sequences the complementary strand for improved accuracy.

Intended Uses and Specific Applications

The DNBSEQ-T1+ provides several enhancements over previous instruments.

  • Throughput and Run Times: With dual FCL flow cells, the DNBSEQ-T1+ can generate up to 1.2 Tb of data in under 24 hours (for PE150 reads). The FCL flow cell alone can produce up to 600 Gb, the FCM up to 300 Gb, and the FCS up to 300 Gb (PE300).
  • Intended Uses: The instrument's flexibility in throughput and read length makes it suitable for a wide array of applications, including:
    • Whole Genome Sequencing (WGS)
    • Whole Exome Sequencing (WES)
    • Transcriptomics (RNA-Seq)
    • Epigenomics (WGBS)
    • Targeted Sequencing
    • Metagenomics and Microbiome Studies
    • Single-Cell Sequencing
    • Spatial Transcriptomics

MGI offers its own suite of library preparation kits compatible with the DNBSEQ-T1+ platform, designed to leverage the DNBSEQ technology. These include Universal Sequencing Reaction Kits for various read lengths and application-specific kits.

Comparison with Competing Instruments

When comparing the DNBSEQ-T1+ with other sequencers, several trade-offs emerge.

  • DNBSEQ-T7: The T7 is a significantly higher-throughput system from MGI, capable of 1-6 Tb of data per day. While its cost per gigabase is lower at capacity, the DNBSEQ-T1+ offers a more moderate throughput and a lower upfront cost, making it accessible for labs that don't need the T7's immense capacity.
  • Illumina NextSeq 1000/2000: Illumina's comparable benchtop sequencers. The DNBSEQ-T1+ can achieve significantly higher output per run (1.2 Tb) than the NextSeq 1000/2000 (up to 540 Gb). MGI often highlights its high Q40 data quality and lower running costs, while Illumina's platforms benefit from a larger ecosystem of validated library prep kits and bioinformatics tools.
  • Illumina NovaSeq X Plus: Illumina's flagship ultra-high-throughput system. The NovaSeq X Plus can generate up to 16 Tb per run, far exceeding the DNBSEQ-T1+'s 1.2 Tb. The NovaSeq X Plus has a very high upfront cost and is designed for the highest-throughput demands of large genome centers, whereas the DNBSEQ-T1+ is a more manageable mid-to-high-throughput option.

Special Considerations

The DNBSEQ-T1+ is a compelling option for certain research groups.

  • Cost-Effectiveness and Affordability: For labs with increasing sequencing demands that cannot justify the capital expenditure of ultra-high-throughput systems, the DNBSEQ-T1+ offers an attractive balance of output and affordability.
  • Workflow Efficiency: The integrated DNB Make & Load module and optional bioinformatics module reduce manual intervention and turnaround time, which is valuable for labs seeking higher automation.
  • Reliable Data: DNBSEQ technology's low rates of index hopping and low duplication rates are beneficial for applications where these factors are critical, leading to more reliable data and higher effective sequencing depth.
  • Flexibility: The ability to choose between three flow cell sizes and run dual flow cells with individually addressable lanes provides unparalleled flexibility for labs with diverse project needs.

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This information on this page is up-to-date as of August 7, 2025 and based on the spec sheets published by the manufacturer.