Complete Genomics DNBSEQ-G800

DNBSEQ-G800 is a sequencer released by Complete Genomics in February 2024. It is based on DNA Nanoball Sequencing.

Complete Genomics DNBSEQ-G800
Photo of DNBSEQ-G800: https://www.completegenomics.com/products/sequencing-platforms/dnbseq-g800/g800-brochure/ from MGI

Specifications

Manufacturer Complete Genomics
Technology DNA Nanoball Sequencing
Release date February 2024
Price [2]
Type Benchtop
Weight
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length
Run time
Reagent cost per Gb [1]
Data quality [3]
Maximum output per run (Gb)[4]
Primary error type [5] Substitutions
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the DNBSEQ-G400

The DNBSEQ-G800, developed by MGI Tech, is a high-throughput next-generation sequencing (NGS) platform targeting the mid- to high-throughput sequencing market. It sits within MGI's portfolio between the DNBSEQ-G400 and their ultra-high throughput platforms, like the DNBSEQ-T7.

Key Innovations

The DNBSEQ-G800 builds upon MGI's core DNB (DNA Nanoball) technology. Key innovations include improvements to the fluidics system, optical system, and data processing pipeline. Many of the improvements come from the incorporation of MGI's CoolMPS sequencing chemistry. This innovative approach uses unlabeled nucleotides and fluorescently labeled antibodies to identify incorporated bases. By using antibodies linked to 2-4 fluorescent dyes instead of fluorescently labeled nucleotides, CoolMPS can reduce background noise and improve signal-to-noise ratio, leading to better imaging and more accurate and longer reads. Additionally, CoolMPS may enable the use of natural nucleotides, which could further improve sequencing accuracy and reduce costs.

Features and Technologies

The DNBSEQ-G800 supports paired-end sequencing and offers flexible run modes, allowing users to adjust the output and run time based on their specific project needs on 2 independent flow cells with 8 lanes each. Data quality metrics for the G800 typically see Q40 accuracy, especially for read lengths of 150 bp and below. Run times vary depending on the chosen run mode and read length, but are generally competitive with other high-throughput platforms, especially the DNBSEQ-G400.

In addition to paired-end sequencing, the DNBSEQ-G800 can also perform single-read sequencing with read lengths up to 600 bp, which is the longest single read among the short-read sequencers on the market. This capability is due to the CoolMPS technology, and it makes the platform suitable for a wider range of applications, including the sequencing of highly variable or repetitive regions, 16S sequencing, and full-length RNA sequencing.

The DNBSEQ-G800 also features an integrated auto-wash module, where the wash cycle will automatically begin after sequencing if a cleaning cartridge is available.

Intended Uses and Specific Applications

The DNBSEQ-G800 is suitable for a wide range of NGS applications, including:

  • Whole-genome sequencing (WGS): Suitable for de novo assembly, variant calling, and structural variation detection.
  • Transcriptome sequencing (RNA-Seq): Enables gene expression profiling, isoform discovery, and analysis of non-coding RNAs.
  • Targeted sequencing: Allows for deep sequencing of specific genomic regions of interest, such as exomes or gene panels.
  • Metagenomic sequencing: Facilitates the study of complex microbial communities, especially when sequencing the 16S region.

MGI offers a variety of library preparation kits compatible with the DNBSEQ-G800 for different applications, including the MGIEasy FS DNA Library Prep Set. Then users can also adapt third-party library preparation kits.

Comparison with Competing Instruments

The DNBSEQ-G800 competes with other high-throughput sequencing platforms, including:

  • Complete Genomics DNBSEQ-G400: The DNBSEQ-G400 can be thought of as MGI's predecessor to the DNBSEQ-G800, as it provides a higher throughput and improved data quality compared to the DNBSEQ-G400, and there is no significant difference between the run times. The G800 is overall an improvement, but if the G400 is already available, buying a new G800 may not be necessary.
  • Illumina NovaSeq 6000: The NovaSeq 6000 is a widely adopted high-throughput sequencer known for its high data quality and relatively fast run times. While the NovaSeq 6000 generally offers a higher output and faster run times than the DNBSEQ-G800, the DNBSEQ-G800 offers higher accuracy and the ability to sequence at 1x600 bp.
  • Illumina NovaSeq X Plus: The NovaSeq X Plus is Illumina's latest high-throughput platform, offering even higher throughput and faster run times compared to the NovaSeq 6000 and the DNBSEQ-G800. However, the DNBSEQ-G800 can offer higher accuracy and single read sequencing up to 600 bp.

Special Considerations

Researchers should consider the DNBSEQ-G800 when:

  • Mid-to-high throughput is needed
  • High short-read accuracy is a priority
  • Sequencing accurately at 1x600 bp could be useful (e.g. genome assembly, 16S sequencing, etc.)

Need sequencing done on a Complete Genomics DNBSEQ-G800 or a specific instrument? Tell us about your project here, and we'll connect you with a qualified provider.

This information on this page is up-to-date as of January 30, 2025 and based on the spec sheets published by the manufacturer.