Illumina MiSeq i100

MiSeq i100 is a sequencer released by Illumina in March 2025. It is based on Sequencing by Synthesis (SBS).

Illumina MiSeq i100
Photo of MiSeq i100: https://www.illumina.com/systems/sequencing-platforms/miseq-i100/specifications.html by Illumina

Specifications

Manufacturer Illumina
Technology Sequencing by Synthesis (SBS)
Release date March 2025
Price [2] $49,000
MSRP as of October 2024
Type Benchtop
Weight 36 Kg
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length
Run time
Reagent cost per Gb [1]
Data quality [3]
Maximum output per run (Gb)[4]
Primary error type [5] Substitutions
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

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This information on this page is up-to-date as of October 17, 2024 and based on the spec sheets published by the manufacturer.