Ultima Genomics UG 100

UG 100 is a sequencer released by Ultima Genomics in February 2024. It is based on Ultima Genomics flow-based chemistry.

Ultima Genomics UG 100
Image of UG 100: https://www.ultimagenomics.com/ug-100/ by Ultima Genomics


Manufacturer Ultima Genomics
Technology Ultima Genomics flow-based chemistry
Release date February 2024
Price [2] $1,500,000
MSRP as of release date
Type Industrial
Weight 771 Kg
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length Ultima Genomics UG 100: 225bp to 300bp+
Run time Ultima Genomics UG 100: 12 to 14 hours
Reagent cost per Gb [1] Ultima Genomics UG 100: Unavailable
Data quality [3] Ultima Genomics UG 100: ≥ 85% flows above Q30
Maximum output per run (Gb)[4] Ultima Genomics UG 100: 2400.00
Primary error type [5] Indels
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the UG 100

The Ultima Genomics UG 100, released in 2024, is a high-throughput next-generation sequencing (NGS) platform aimed at disrupting the high-throughput sequencing market segment by theoretically offering the $100 genome. It is not a direct successor to any specific older instrument but represents a significant departure from traditional sequencing technologies with its unique architecture and chemistry.

Key Innovations

The UG 100's core innovation lies in its use of open silicon wafers instead of traditional flow cells. This shift enables a massive increase in sequencing reaction surface area, leading to higher throughput and lower costs.

These innovations are achieved through a combination of hardware and software engineering. The use of circular wafers allows for efficient reagent use and high-speed scanning of large surfaces, and the 24/7 automation makes the process much easier for lab staff.

Machine learning algorithms are also employed to analyze and interpret the vast amounts of data generated by the platform, which results in high single nucleotide variant (SNV) accuracy.

Features and Technologies

  • Open Substrate: Eliminates the need for costly flow cells and simplifies fluidics.
  • Circular Wafers: Enables efficient reagent use, zero crosstalk, and high-speed scanning.
  • Continuous Operation: Enables continuous operation through automated wafer handling for high throughput and minimal hands-on time.
  • Data Quality Metrics: Offers high base-calling accuracy and low error rates comparable to other high-throughput sequencers. It can generate tens of billions of reads per run, with run times under 14 hours.

Intended Uses and Specific Applications

The UG 100 is suitable for various NGS applications, including:

  • Whole-Genome Sequencing (WGS): Comprehensive analysis of entire genomes. Multiple IDT xGen for Ultima Genomics reagents are suitable here.
  • RNA Sequencing (RNA-Seq): Analysis of gene expression and transcriptomes. The NEBNext Ultra II RNA Library Prep Kit can be used here.
  • Single-Cell Sequencing: Study of individual cells within a population. 10X Genomics kits are compatible for this application.
  • Methylation Sequencing: Analysis of epigenetic modifications. The IDT xGen Methyl-Seq DNA Library Prep Kit is suitable for this application.

Comparison with Competing Instruments

  • Complete Genomics DNBSEQ-G400: The DNBSEQ-G400 offers paired-end reads and has a lighter footprint than the UG100. However, the UG 100 offers the potential for longer read lengths, significantly faster run times and higher output.
  • Illumina NovaSeq 6000: The NovaSeq 6000 has a lower upfront cost and lighter weight and can offer long paired-end reads. However, the UG 100 offers the potential for longer read lengths, faster run times especially on the higher output end and higher max output, except when compared with the S4.

Special Considerations

The UG100's unique architecture and chemistry may require adjustments to existing workflows and analysis pipelines. However, its potential for cost reduction and increased throughput makes it an attractive option for researchers seeking to scale up their sequencing efforts.

Need sequencing done on a Ultima Genomics UG 100 or a specific instrument? Tell us about your project here, and we'll connect you with a qualified provider.

This information on this page is up-to-date as of May 13, 2024 and based on the spec sheets published by the manufacturer.