Complete Genomics DNBSEQ-T20X2

DNBSEQ-T20X2 is a sequencer released by Complete Genomics in February 2023. It is based on DNA Nanoball Sequencing.

Complete Genomics DNBSEQ-T20X2
Image of DNBSEQ-T20X2: by MGI


Manufacturer Complete Genomics
Technology DNA Nanoball Sequencing
Release date February 2023
Price [2]
Type Industrial
Weight 3700 Kg
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length Complete Genomics DNBSEQ-T20X2: 2x100bp to 2x150bp
Run time Complete Genomics DNBSEQ-T20X2: 60 to 80 hours
Reagent cost per Gb [1] Complete Genomics DNBSEQ-T20X2: Unavailable
Data quality [3] Complete Genomics DNBSEQ-T20X2: ≥ 85% bases above Q30 for PE100
Maximum output per run (Gb)[4] Complete Genomics DNBSEQ-T20X2: 72000.00
Primary error type [5] Substitutions
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the DNBSEQ-T20x2

The DNBSEQ-T20x2 from Complete Genomics, a subsidiary of MGI, is an ultra-high throughput next-generation sequencing (NGS) instrument positioned for large-scale, production-level genomic projects. It builds upon the same core technology as its predecessors in the DNBSEQ lineup, offering significant improvements in throughput and cost efficiency. If your research involves large population genome studies, building extensive genomic databases, or requires very high sequencing output, the DNBSEQ-T20x2 warrants serious consideration.

Key Innovations

The DNBSEQ-T20x2's key advancements center around significantly increased throughput and reduced sequencing costs:

  • Robotic Automation: The DNBSEQ-T20x2 incorporates a sophisticated robotic system that automates various steps throughout the sequencing process, including library loading, reagent handling, and slide washing. This extensive automation minimizes hands-on time by lab personnel, reducing the risk of human error and improving overall workflow consistency. Additionally, automation helps to ensure standardized processing conditions across all samples, leading to more reliable and reproducible sequencing results.
  • Increased Slide Capacity: The DNBSEQ-T20x2 can process up to six slides per run, a higher number than the previous models in the DNBSEQ lineup. The ability to process more slides per run translates to a significant boost in sequencing output. Researchers can sequence more samples per run, reducing total run time and costs required to achieve a desired level of coverage.
  • Dip-Immersion Technology: DNBSEQ-T20x2 utilizes a novel dip-immersion flow cell design that reduces reagent consumption. In traditional flow cell sequencing, reagents flow over the surface of the slide. In dip-immersion technology, the slide is submerged in a trough containing the sequencing reagents. This reduces the amount of reagent required per sample, as the reagents are constantly replenished during the sequencing run. The T20x2’s dip-immersion system precisely controls the volume of reagent used and minimizes reagent waste. This technology contributes to significant cost savings, particularly for high-throughput sequencing projects.

Features and Technologies

  • DNBseq Sequencing: Complete Genomics’s core sequencing technology uses DNA nanoballs (DNBs) for template generation, combined with combinatorial probe anchor synthesis (cPAS) sequencing chemistry. This technology offers high accuracy and flexibility.
  • Ultra-High Throughput: The DNBSEQ-T20x2 can produce up to 72 Tb of data per run, enabling sequencing of around 50,000 whole human genomes (30x coverage) annually.
  • Suitable Read Lengths: Supports read lengths that are the most common configuration for whole-genome sequencing, PE100 and PE150.

Intended Uses and Specific Applications

  • Whole-Genome Sequencing (WGS): Ideal for large-scale population studies, disease association research, and the discovery of novel genetic variants. Compatible with standard third-party WGS library prep kits if using Complete Genomics library conversion kits.
  • Exome Sequencing: Suitable for targeted sequencing of protein-coding regions. Compatible with standard third-party WES library prep kits if using Complete Genomics library conversion kits.
  • Transcriptome Sequencing (RNA-seq): Can be used for gene expression profiling, transcript discovery, and differential expression analysis. Compatible with standard third-party RNA-Seq library prep kits if using Complete Genomics library conversion kits.

Comparison with Competing Instruments

  • Complete Genomics DNBSEQ-T7: The T7 offers high-output but certainly not to the extent of the T20x2. However, the T7 run times are much faster and may be more affordable for most labs.
  • Illumina NovaSeq X: The NovaSeq X offers high output, although again not to the level of the DNBSEQ-T20x2, and the NovaSeq X is also slightly more accurate with faster run times, especially with the 1.5B and 10B flow cells.

Special Considerations

The DNBSEQ-T20x2 is best suited for research projects that require ultra-high throughput sequencing and have a high sample volume to justify the instrument's capacity. Examples include large population genomics studies, large-scale cancer genomics studies, and functional genomics projects requiring deep whole-genome sequencing of many samples.

Need sequencing done on a Complete Genomics DNBSEQ-T20X2 or a specific instrument? Tell us about your project here, and we'll connect you with a qualified provider.

This information on this page is up-to-date as of April 15, 2024 and based on the spec sheets published by the manufacturer.