Complete Genomics DNBSEQ-G99

DNBSEQ-G99 is a sequencer released by Complete Genomics in November 2022. It is based on DNA Nanoball Sequencing.

Complete Genomics DNBSEQ-G99
Image of G99: https://en.mgi-tech.com/news/340/ by MGI

Specifications

Manufacturer Complete Genomics
Technology DNA Nanoball Sequencing
Release date November 2022
Price [2]
Type Benchtop
Weight 140 Kg
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length Complete Genomics DNBSEQ-G99: 1x100bp to 2x150bp
Run time Complete Genomics DNBSEQ-G99: 5 to 12 hours
Reagent cost per Gb [1] Complete Genomics DNBSEQ-G99: Unavailable
Data quality [3] Complete Genomics DNBSEQ-G99: ≥ 90% bases above Q30 for SE100 and PE50
Maximum output per run (Gb)[4] Complete Genomics DNBSEQ-G99: 48.00
Primary error type [5] Substitutions
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the DNBSEQ-G99

The DNBSEQ-G99 is a next-generation sequencing (NGS) platform from MGI Tech designed for the mid- to low-output segment of the NGS market. It is known for its exceptional speed, making it a compelling choice for researchers focusing on targeted gene panels, small genome sequencing, and applications requiring rapid turnaround times.

Key Innovations

The DNBSEQ-G99 achieves its impressive speed through several key innovations:

  • Triangular Matrix Signal Spots: The flow cell utilizes a novel triangular matrix design for capturing sequencing data. This innovative approach replaces the traditional square grid of sequencing spots with a denser arrangement of triangular spots. By packing more sequencing spots onto the flow cell surface area, the DNBSEQ-G99 can capture significantly more sequence data in a single run compared to instruments using square grids. This translates to a substantial increase in throughput without requiring a larger flow cell or more complex optics.
  • Ultra-Simple Cassette Flow Cell: The redesigned flow cell simplifies loading and reduces the risk of errors during sample preparation. Its larger safe holding area minimizes potential sample loss.
  • Innovative Reagent Pre-Injection: The reagent cartridge features a pre-injection design that eliminates the need for manual pipetting steps during reagent preparation. This innovative approach reduces the risk of errors associated with manual handling and saves researchers time. The pre-filled reagent cartridge simply clicks into the instrument, and with a single press, the required volume of reagents is automatically injected into the flow cell. This streamlines the sequencing workflow and minimizes the potential for errors introduced during manual handling steps.

Features and Technologies

The DNBSEQ-G99 leverages MGI's DNBSEQ core sequencing technology, which relies on rolling circle amplification (RCA). It boasts a high data quality with:

  • Base calling accuracy: >Q30 (over 99.9% accuracy)
  • Error rate: <0.1%

The platform offers a turnaround time of just 12 hours for PE150 sequencing, making it significantly faster than many competing instruments in its throughput category.

Furthermore, the DNBSEQ-G99 boasts several user-friendly features:

  • Dual Flow Cell Capability: The system can accommodate two flow cells for independent loading and running, maximizing instrument utilization.
  • Optional Integrated Bioinformatics Module: An optional module allows for on-board data analysis, streamlining the workflow from sequencing to initial results.

Intended Uses and Specific Applications

The DNBSEQ-G99 excels in applications demanding speed and high-quality data for moderate-sized datasets. Here are some of its ideal uses:

  • Targeted Gene Panel Sequencing: The speed and accuracy of the DNBSEQ-G99 make it suitable for analyzing targeted panels of genes associated with specific diseases or traits. Several compatible library preparation kits are available, including the MGIEasy Targeted Capture Probe Sets V4 and V5.
  • Small Genome Sequencing: The platform efficiently sequences the complete genomes of smaller organisms like bacteria, viruses, and fungi. MGI offers specific library preparation kits for this application, such as the MGIEasy Fast FS Library Prep Set V2.0 and the PCR-Free version.
  • Metagenomics: The DNBSEQ-G99 is well-suited for analyzing the genetic makeup of microbial communities, particularly when rapid turnaround times are crucial. MGI offers the MGIEasy Fast FS Library Prep Set V2.0 for metagenomic library preparation.

Comparison with Competing Instruments

  • Complete Genomics DNBSEQ-E25: While the DNBSEQ-E25 shares the DNBSEQ core technology, it offers lower throughput at very similar read lengths, run times and accuracy, making it ideal for very small projects. The choice between this and the DNBSEQ-G99 may come down to upfront cost and how often mid-output vs. low-output sequencing needs to be done.
  • Illumina NextSeq 550: The NextSeq 550 offers a range of output, both similar to and higher than the DNBSEQ-G99, but it utilizes a different sequencing chemistry. Illumina also offers a wider range of library prep options and established bioinformatics pipelines. However, the DNBSEQ-G99 boasts much faster run times, slightly higher accuracy and potentially lower reagent costs.
  • PacBio Onso: PacBio Onso is a short-read sequencer from PacBio. Although the accuracy and output is higher than the DNBSEQ-G99, the G99 is still significantly faster.

Special Considerations

The DNBSEQ-G99 is a strong choice for researchers prioritizing speed and accuracy for targeted resequencing, small genome sequencing, and metagenomics projects. While it offers a competitive price point and streamlined workflow, researchers should consider the availability of specific library preparation kits for their application and the existing bioinformatics infrastructure in their lab.

Need sequencing done on a Complete Genomics DNBSEQ-G99 or a specific instrument? Tell us about your project here, and we'll connect you with a qualified provider.

This information on this page is up-to-date as of April 12, 2024 and based on the spec sheets published by the manufacturer.