Complete Genomics DNBSEQ-E25

DNBSEQ-E25 is a sequencer released by Complete Genomics in August 2022. It is based on DNA Nanoball Sequencing.

Complete Genomics DNBSEQ-E25
Photo of DNBSEQ-E25: from MGI


Manufacturer Complete Genomics
Technology DNA Nanoball Sequencing
Release date August 2022
Price [2]
Type Field
Supports real-time analysis No
Demultiplexing support Yes
File format FASTQ
Read length Complete Genomics DNBSEQ-E25: 1x100bp to 2x150bp
Run time Complete Genomics DNBSEQ-E25: 5 to 20 hours
Reagent cost per Gb [1] Complete Genomics DNBSEQ-E25: Unavailable
Data quality [3] Complete Genomics DNBSEQ-E25: ≥ 90% bases above Q30 for SR100 or shorter
Maximum output per run (Gb)[4] Complete Genomics DNBSEQ-E25: 7.50
Primary error type [5] Substitutions
Notes about the specifications

  1. Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
  2. Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
  3. Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
  4. Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
  5. Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the DNBSEQ-E25

The DNBSEQ-E25, manufactured by MGI Tech, is a compact, benchtop DNA sequencing instrument designed to be exceptionally portable and user-friendly. It's positioned to excel specifically in situations where rapid results or sequencing on location are priorities. Examples might include infectious disease diagnostics or small genome analysis in remote research settings. The DNBSEQ-E25 isn't a direct successor to any previous MGI model but showcases significant advancements over their less portable instruments in terms of size and ease of use.

Key Innovations

  • Integrated Design for Sequencing on the Go: The DNBSEQ-E25 achieves its remarkable portability by consolidating several critical components into a single, self-contained unit. This miniaturization effort includes:
    • Microfluidic flow cell: Reagents can enter the flow cell directly from the reagent cartridge, so no instrument wash is required between runs.
    • Integrated CMOS detection module: A CMOS (complementary metal-oxide-semiconductor) detector chip efficiently detects light signals during the sequencing process without needing more complicated optics.
    • Self-luminous dyes: The dyes don’t need an excitation source in order to emit light, so the need for a bulky external light source during sequencing is eliminated.
    • Onboard computing module: The DNBSEQ-E25 incorporates a dedicated computer for real-time processing and analysis of sequencing data. This eliminates the need for a separate computer and associated software, streamlining the workflow and reducing the overall footprint of the instrument.
  • Rapid Turnaround Time: The DNBSEQ-E25 is designed to prioritize speed without sacrificing accuracy. Depending on the chosen run parameters, sequencing data can be produced in around five hours (SE100 mode). This rapid turnaround time makes the E25 ideal for applications where timely results are critical, such as in outbreak investigations. For instance, in the context of a suspected foodborne illness outbreak, rapid pathogen identification can inform public health interventions and prevent further spread.

Features and Technologies

  • DNBSEQ Sequencing Chemistry: MGI's sequencing platforms utilize DNBSEQ chemistry, characterized by the use of DNA nanoballs (DNBs). DNBs are generated from rolling circle replication, creating dense clusters of identical DNA fragments. Sequencing is then performed by the incorporation of fluorescently labeled nucleotides.
  • Flexible Data Output: The DNBSEQ-E25 produces 2.5–7.5 GB of data per run and can be configured for SE100 or PE150 workflows.
  • Data Quality: MGI reports high accuracy with a low error rate (>99.9%).

Intended Uses and Specific Applications

The DNBSEQ-E25 excels in applications where rapid, on-site results are highly valued:

  • Pathogen Detection & Surveillance: Ideal for microbial identification, outbreak analysis, and rapid diagnostics. Compatible library prep kits include the MGIEasy Fast FS Library Prep Set V2.0 and the PCR-Free version.
  • Targeted Sequencing and Small Genomes: Well-suited for amplicon panels, small viral genomes, or bacterial genomes. Compatible library prep kits include the MGIEasy Fast FS Library Prep Set V2.0 and the PCR-Free version.
  • Field Research: Its compact size and minimal environmental requirements make it a portable option for research in remote locations.

Comparison with Competing Instruments

  • Complete Genomics DNBSEQ-G99: The DNBSEQ-G99 also uses DNBSEQ chemistry but is designed for higher output. It sacrifices portability and speed for greater data output per run.
  • Illumina MiniSeq: The MiniSeq offers comparable ease of use and output, but run times are notably longer than the DNBSEQ-E25, and the MiniSeq is not portable.
  • Illumina MiSeq: The MiSeq offers both higher and lower output than the E25 with a wider read length range, so it can be more suitable for a larger variety of projects. However, the run times are much longer and it’s not portable, like the DNBSEQ-E25.

Special Considerations

The DNBSEQ-E25 offers a compelling use-case if:

  • Fast results take precedence over very high throughput
  • Portability and ease-of-use are critical factors
  • Projects fit within the E25's data output range

Need sequencing done on a Complete Genomics DNBSEQ-E25 or a specific instrument? Tell us about your project here, and we'll connect you with a qualified provider.

This information on this page is up-to-date as of April 12, 2024 and based on the spec sheets published by the manufacturer.