Complete Genomics DNBSEQ-G400

Published on April 12, 2024

DNBSEQ-G400 is a sequencer released by Complete Genomics in October 2022. It is based on DNA Nanoball Sequencing.

Complete Genomics DNBSEQ-G400
Photo of DNBSEQ-G400: https://en.mgi-tech.com/products/instruments_info/2/ by MGI

DNBSEQ-G400 is available in the following models:

DNBSEQ-G400 FAST
DNBSEQ-G400 FCL
DNBSEQ-G400 FCS

Specifications

Manufacturer	Complete Genomics
Technology	DNA Nanoball Sequencing
Release date	October 2022
Price [2]	$
Type	Industrial
Weight	200 Kg
Supports real-time analysis	No
Demultiplexing support	Yes
File format	FASTQ
Read length	Complete Genomics DNBSEQ-G400 FAST: 1x100bp to 2x150bp Complete Genomics DNBSEQ-G400 FCL: 1x50bp to 2x200bp Complete Genomics DNBSEQ-G400 FCS: 1x100bp to 2x300bp
Run time	Complete Genomics DNBSEQ-G400 FAST: 12 to 37 hours Complete Genomics DNBSEQ-G400 FCL: 14 to 107 hours Complete Genomics DNBSEQ-G400 FCS: 13 to 96 hours
Reagent cost per Gb [1]	Complete Genomics DNBSEQ-G400 FAST: Unavailable Complete Genomics DNBSEQ-G400 FCL: Unavailable Complete Genomics DNBSEQ-G400 FCS: Unavailable
Data quality [3]	Complete Genomics DNBSEQ-G400 FAST: ≥ 85% bases above Q30 for PE100 or shorter Complete Genomics DNBSEQ-G400 FCL: ≥ 85% bases above Q30 for PE150 or shorter Complete Genomics DNBSEQ-G400 FCS: ≥ 85% bases above Q30
Maximum output per run (Gb)[4]	Complete Genomics DNBSEQ-G400 FAST: 330.00 Complete Genomics DNBSEQ-G400 FCL: 720.00 Complete Genomics DNBSEQ-G400 FCS: 180.00
Primary error type [5]	Substitutions

Notes about the specifications

Cost per Gb: The cost per Gb is calculated by dividing the costs of sequencing reagents by the manufacturer’s maximum output per run. For these runs, the manufacturer may have included their own controls and used specific library preparation kits. Actual performance may vary based on sample and library type and quality, loading concentration, and other experimental factors. The sequencing reagent costs used for calculations do not include any other costs associated with preparing the samples or running the instrument, including reagents used for extraction, quality control or library preparation, labor, electricity, lab consumables, storage, etc. Performance metrics and prices are subject to change.
Price: The final price may be different depending on the supplier, the region and any discounts provided through bulk orders or negotiation. Other factors that may influence the final price are warranty options, maintenance contracts, possible trade-ins of older instruments, and any financing arrangements chosen.
Data quality: This is the maximum data quality that the instrument can offer based on manufacturer’s specifications and optimizations. The quality of a specific run will depend on multiple factors, including sample quality, sample quantity, library prep protocol, run conditions, etc.
Maximum output: This is the maximum output of a full instrument run. This number is based on manufacturer’s specifications and optimizations.
Primary error type: This is the most common type of error that may occur due to sequencing. Other types of error may occur as well.

More about the DNBSEQ-G400

The DNBSEQ-G400, manufactured by MGI Tech (a subsidiary of BGI Group), is a high-throughput next-generation sequencing (NGS) instrument built upon the company's proprietary DNA Nanoball (DNB) sequencing technology. This platform finds its primary place in the high-output segment of the NGS market, making it suitable for large-scale sequencing projects ranging from whole-genome and whole-exome sequencing to targeted sequencing and transcriptomics.

Key Innovations

High Data Output: The DNBSEQ-G400 delivers a considerable data output, ranging from 55 Gb to a staggering 1440 Gb per run. This scalability is achieved through the use of two distinct flow cell types: the FCS (Flow Cell Standard) and the FCL (Flow Cell Large). The FCS flow cell is ideal for projects requiring moderate throughput, while the FCL caters to large-scale sequencing needs. This flexibility allows researchers to optimize their workflow and costs based on the specific demands of their project. For instance, researchers performing targeted resequencing panels or transcriptome sequencing on a smaller number of samples might opt for the FCS, while those undertaking whole-genome sequencing of multiple samples or large RNA-seq studies may benefit from the increased capacity of the FCL.
Enhanced Read Lengths: Supporting read lengths of up to PE300 (paired-end 300), the G400 provides improved capabilities in applications like de novo genome assembly and structural variant detection.
Improved Optical and Biochemical Systems: The DNBSEQ-G400 incorporates advancements in both the instrument's optical systems and the underlying biochemistry of the sequencing process. This synergistic approach leads to several user benefits:
- Reduced Run Times: By optimizing the efficiency of the sequencing chemistry and enhancing the image capture and processing pipeline, MGI has significantly reduced run times compared to previous models. For instance, PE150 sequencing can be completed in approximately 37 hours, making the G400 a competitive choice for researchers requiring fast turnaround times for their projects.
- Streamlined Workflow: The G400's design prioritizes user experience. The instrument boasts simplified library preparation protocols and automated processes that minimize manual intervention, leading to a more streamlined sequencing workflow. This translates to reduced hands-on time and the potential for fewer errors.

Features and Technologies

DNBSEQ Sequencing: The G400 utilizes DNBSEQ technology, where fragmented DNA is circularized, then amplified through rolling circle replication to form DNA Nanoballs (DNBs). These DNBs are loaded onto patterned nanoarrays, followed by sequenced using combinatorial probe-anchor synthesis (cPAS).
Flexible Flow Cell Configurations: Researchers can choose between two flow cell types (FCS and FCL), offering a dynamic range of sequencing output to fit various project needs and budgets.
Primary Data Analysis: The G400 includes an integrated primary analysis module that streamlines the sequencing workflow by providing automated base calling, quality control (QC) metrics generation, and initial data processing steps. This can significantly reduce analysis time and bioinformatics expertise required for researchers, particularly those new to NGS. The G400's software suite also offers data visualization tools for a preliminary grasp of the sequencing data. However, for in-depth secondary analysis and variant calling, researchers will likely need to utilize additional third-party software or MGI's more comprehensive analysis pipelines.

Intended Uses and Applications

The DNBSEQ-G400 supports a wide array of NGS applications, including:

Whole-Genome Sequencing (WGS): High-depth sequencing for comprehensive variant discovery with suitable library prep kits (e.g., MGIEasy PCR-Free DNA Library Prep Set)
Whole-Exome Sequencing (WES): Cost-effective analysis of protein-coding regions, often with specific target enrichment kits, such as the MGIEasy Exome FS Library Prep Set.
Transcriptome Sequencing (RNA-seq): Studies of gene expression, alternative splicing, etc. Library prep kits such as MGIEasy RNA Library Prep Set are well-suited.

Comparison with Competing Instruments

Complete Genomics DNBSEQ-T7: Similar DNB-based technology. The T7 offers higher output at similar accuracy and faster run times, but it is restricted to a max 150 bp read length and is much heavier.
Ultima Genomics UG100: The UG100, while offering higher max output at similar read lengths, may have a higher initial instrument purchase price and is also much heavier than the DNBSEQ-G400. Ultima focuses heavily on very low per-base sequencing costs for large studies.
Element Biosciences AVITI: Although it has a lower max output for similar read lengths, it has generally faster run times and a potentially lower cost of entry. Its technology is newer, and data quality metrics are still evolving.

Special Considerations

The DNBSEQ-G400 offers a compelling combination of high throughput, accuracy, and flexibility for researchers. Factors favoring its selection include:

Established Technology: DNBSEQ is quickly becoming a proven technology with a track record of successful use in numerous studies.
Cost-effectiveness: Competitive reagent costs contribute to the G400's potential for cost-effective high-throughput sequencing.
Ecosystem: MGI offers a range of library preparation kits and analysis solutions, facilitating integration into research workflows.

Need sequencing done on a Complete Genomics DNBSEQ-G400 or a specific instrument? Tell us about your project here, and we'll connect you with a qualified provider.

This information on this page is up-to-date as of April 12, 2024 and based on the spec sheets published by the manufacturer.